The defender made a long-awaited return to the field after being sidelined for 14 months with a knee injury.
Mings played the first 45 minutes for the U21s against Newcastle United.
Midfielder Boubacar Kamara also featured after he had recovered from a similar ACL setback suffered in February.
Villa boss Unai Emery has hopes of Mings and Kamara being ready for first team football after the international break.